orthogonal ni- column (25 genscript high affinity ni- charged resin) Search Results


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GenScript corporation high-affinity ni-charged resin ff (l00666-25)
High Affinity Ni Charged Resin Ff (L00666 25), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation ni-column
Ni Column, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation ni-affinity column
Ni Affinity Column, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation gravity ni-nta column
Gravity Ni Nta Column, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation orthogonal ni- column (25 genscript high affinity ni- charged resin)
Orthogonal Ni Column (25 Genscript High Affinity Ni Charged Resin), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation ni-charged magbeads
Ni Charged Magbeads, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation ni-charged resin
Ni Charged Resin, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation chromatography column
Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion <t>chromatography</t> of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)
Chromatography Column, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation toxinsensor gel clot endotoxin assay kit
Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion <t>chromatography</t> of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)
Toxinsensor Gel Clot Endotoxin Assay Kit, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation ni-nta resin genescript
Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion <t>chromatography</t> of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)
Ni Nta Resin Genescript, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation high-affinity ni-nta resin
Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion <t>chromatography</t> of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)
High Affinity Ni Nta Resin, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high-affinity ni-nta resin - by Bioz Stars, 2026-06
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GenScript corporation 6xhistagged pkm2 protein
Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion <t>chromatography</t> of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)
6xhistagged Pkm2 Protein, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion chromatography of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)

Journal: BMC Microbiology

Article Title: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis

doi: 10.1186/s12866-020-02014-z

Figure Lengend Snippet: Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion chromatography of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)

Article Snippet: The supernatant was passed through a 0.45 μm filter and loaded on a chromatography column containing 5 mL of Ni 2+ -agarose resin (Genscript) pre-equilibrated with 25 mL of buffer A.

Techniques: Purification, SDS Page, Marker, Size-exclusion Chromatography, Molecular Weight

Fe-S cluster reconstitution of purified IscU2. a SDS-PAGE analysis of purified IscU2 (cropped image of original). Lane 1, MW marker; lane 2, IscU2 (2.4 μg). b Anaerobic UV-visible spectra of 20 μM IscU2 (dotted line), IscU2 S-FeS (black line) and IscU2 C-FeS (gray line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Anaerobic size exclusion chromatography of 14.8 mg of IscU2 (gray line) and 15.6 mg of IscU2 C-FeS (black line) in 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscU2 and IscU2 C-FeS were calculated with a standard curve (inset). The calculated MW of IscU2 (solid square symbol) was 12 kDa and the calculated MW of IscU2 C-FeS (open square symbol) was 26 kDa

Journal: BMC Microbiology

Article Title: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis

doi: 10.1186/s12866-020-02014-z

Figure Lengend Snippet: Fe-S cluster reconstitution of purified IscU2. a SDS-PAGE analysis of purified IscU2 (cropped image of original). Lane 1, MW marker; lane 2, IscU2 (2.4 μg). b Anaerobic UV-visible spectra of 20 μM IscU2 (dotted line), IscU2 S-FeS (black line) and IscU2 C-FeS (gray line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Anaerobic size exclusion chromatography of 14.8 mg of IscU2 (gray line) and 15.6 mg of IscU2 C-FeS (black line) in 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscU2 and IscU2 C-FeS were calculated with a standard curve (inset). The calculated MW of IscU2 (solid square symbol) was 12 kDa and the calculated MW of IscU2 C-FeS (open square symbol) was 26 kDa

Article Snippet: The supernatant was passed through a 0.45 μm filter and loaded on a chromatography column containing 5 mL of Ni 2+ -agarose resin (Genscript) pre-equilibrated with 25 mL of buffer A.

Techniques: Purification, SDS Page, Marker, Size-exclusion Chromatography, Molecular Weight