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Image Search Results
Journal: BMC Microbiology
Article Title: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis
doi: 10.1186/s12866-020-02014-z
Figure Lengend Snippet: Purified IscS2 binds PLP and is a homodimer. a SDS-PAGE analysis of purified IscS2 (cropped image of original). Lane 1, MW marker; lane 2, IscS2 (2.5 μg). b UV-visible spectra of 20 μM IscS2 (dotted line) or IscS2 PLP (solid line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Size-exclusion chromatography of IscS2. IscS2 (3.3 mg loaded) was analyzed by size-exclusion chromatography with 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscS2 was calculated from a standard curve (inset). The square represents the V e /V o of IscS2 with a calculated molecular weight of 84 kDa, consistent with homodimer (87 kDa)
Article Snippet: The supernatant was passed through a 0.45 μm filter and loaded on a
Techniques: Purification, SDS Page, Marker, Size-exclusion Chromatography, Molecular Weight
Journal: BMC Microbiology
Article Title: Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis
doi: 10.1186/s12866-020-02014-z
Figure Lengend Snippet: Fe-S cluster reconstitution of purified IscU2. a SDS-PAGE analysis of purified IscU2 (cropped image of original). Lane 1, MW marker; lane 2, IscU2 (2.4 μg). b Anaerobic UV-visible spectra of 20 μM IscU2 (dotted line), IscU2 S-FeS (black line) and IscU2 C-FeS (gray line) in 50 mM Tris pH 7.2, 150 mM NaCl. c Anaerobic size exclusion chromatography of 14.8 mg of IscU2 (gray line) and 15.6 mg of IscU2 C-FeS (black line) in 50 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT, 10% glycerol. The molecular weight of IscU2 and IscU2 C-FeS were calculated with a standard curve (inset). The calculated MW of IscU2 (solid square symbol) was 12 kDa and the calculated MW of IscU2 C-FeS (open square symbol) was 26 kDa
Article Snippet: The supernatant was passed through a 0.45 μm filter and loaded on a
Techniques: Purification, SDS Page, Marker, Size-exclusion Chromatography, Molecular Weight